bbsi site (Addgene inc)
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Addgene inc
bbsi site
Bbsi Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+sites/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/bio_rxiv__64898__2026__01__22__701170-240-27-29
Average 96 stars, based on 4065 article reviews
Bbsi Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+sites/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/bio_rxiv__64898__2026__01__22__701170-240-27-29
Average 96 stars, based on 4065 article reviews
bbsi site - by Bioz Stars,
2026-09
96/100 stars
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CRISPR:Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the Article Title: Restored Collagen VI Microfilaments Network in the Extracellular Matrix of CRISPR-Edited Ullrich Congenital Muscular Dystrophy Fibroblasts. Article Snippet: .. The CRISPR/Cas9 effector plasmids for gRNA1 and gRNA3 were generated by cloning two annealed oligonucleotides for each gRNA into Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication. Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the Cloning:Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the Article Title: Restored Collagen VI Microfilaments Network in the Extracellular Matrix of CRISPR-Edited Ullrich Congenital Muscular Dystrophy Fibroblasts. Article Snippet: .. The CRISPR/Cas9 effector plasmids for gRNA1 and gRNA3 were generated by cloning two annealed oligonucleotides for each gRNA into Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication. Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the Sequencing:Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the Article Title: The role and mechanism of p53 F229V mutation in inhibiting pseudorabies virus replication. Article Snippet: .. The CRISPR/Cas9 targeting plasmids were assembled by cloning annealed oligonucleotides encoding the desired sgRNAs into the Article Title: Subcellular transcriptome of radial glia reveals compartmentalized control of cortical development Article Snippet: .. U6-gRNA: Addgene #109053. gRNAs were annealed and inserted using built in BbsI sites. pCAG-gRNA: The RfxCas13d DR30 sequence and Phospho-proteomics:Article Title: Identification of the Staphylococcus aureus Endothelial Cell Surface Interactome by Proximity Labeling Article Snippet: Generation of K.O. cell lines is based on a previous protocol [ ]. sgRNAs ( ) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. No. ER0031), the sgRNAs were cloned into the Article Title: Identification of the Staphylococcus aureus endothelial cell surface interactome by proximity labeling Article Snippet: Single guide RNAs (sgRNAs; ) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. no. ER0031), the sgRNAs were cloned into the Article Title: Identification of the Staphylococcus aureus endothelial cell surface interactome by proximity labeling. Article Snippet: Single guide RNAs (sgRNAs; Table 1) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. no. ER0031), the sgRNAs were cloned into the Clone Assay:Article Title: Identification of the Staphylococcus aureus Endothelial Cell Surface Interactome by Proximity Labeling Article Snippet: Generation of K.O. cell lines is based on a previous protocol [ ]. sgRNAs ( ) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. No. ER0031), the sgRNAs were cloned into the Article Title: Identification of the Staphylococcus aureus endothelial cell surface interactome by proximity labeling Article Snippet: Single guide RNAs (sgRNAs; ) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. no. ER0031), the sgRNAs were cloned into the Article Title: Essential roles of CTCF binding sites at TAD boundaries in modulating chromatin interactions and transcriptional regulation at the Ifng locus. Article Snippet: .. Briefly, the following pairs of single-guide RNA (sgRNA) sequences were annealed and cloned into the Article Title: Identification of the Staphylococcus aureus endothelial cell surface interactome by proximity labeling. Article Snippet: Single guide RNAs (sgRNAs; Table 1) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. no. ER0031), the sgRNAs were cloned into the Ligation:Article Title: Identification of the Staphylococcus aureus Endothelial Cell Surface Interactome by Proximity Labeling Article Snippet: Generation of K.O. cell lines is based on a previous protocol [ ]. sgRNAs ( ) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. No. ER0031), the sgRNAs were cloned into the Article Title: Identification of the Staphylococcus aureus endothelial cell surface interactome by proximity labeling Article Snippet: Single guide RNAs (sgRNAs; ) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. no. ER0031), the sgRNAs were cloned into the Article Title: Identification of the Staphylococcus aureus endothelial cell surface interactome by proximity labeling. Article Snippet: Single guide RNAs (sgRNAs; Table 1) were designed with the CHOPCHOP online tool (chopchop.cdu.uib.no) and synthesized as primer pairs (Sigma Aldrich). .. After phosphorylation with T4 nucleotide kinase (ThermoFisher, Cat. no. ER0031), the sgRNAs were cloned into the Plasmid Preparation:Article Title: Essential roles of CTCF binding sites at TAD boundaries in modulating chromatin interactions and transcriptional regulation at the Ifng locus. Article Snippet: .. Briefly, the following pairs of single-guide RNA (sgRNA) sequences were annealed and cloned into the Article Title: Restored Collagen VI Microfilaments Network in the Extracellular Matrix of CRISPR-Edited Ullrich Congenital Muscular Dystrophy Fibroblasts. Article Snippet: .. The CRISPR/Cas9 effector plasmids for gRNA1 and gRNA3 were generated by cloning two annealed oligonucleotides for each gRNA into Generated:Article Title: Restored Collagen VI Microfilaments Network in the Extracellular Matrix of CRISPR-Edited Ullrich Congenital Muscular Dystrophy Fibroblasts. Article Snippet: .. The CRISPR/Cas9 effector plasmids for gRNA1 and gRNA3 were generated by cloning two annealed oligonucleotides for each gRNA into Amplification:Article Title: Subcellular transcriptome of radial glia reveals compartmentalized control of cortical development Article Snippet: .. U6-gRNA: Addgene #109053. gRNAs were annealed and inserted using built in BbsI sites. pCAG-gRNA: The RfxCas13d DR30 sequence and |